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Dr. Shiaw-Lin Wu Northwestern University, U.S.A E-mail : si.wu@neu.edu Web-site : |
Dr. Wu spent his early career in biotechnology industry (Genentech) where he learned protein biochemistry and applications of analytical bioscience, and then later in the mass spectrometry company (ThermoFinnigan) where he learned the ion trap, fourier transform mass spectrometry, and the study of proteomics using mass spectrometry approach. Recently, he joined the Barnett Institute of Northeastern University to develop LC-MS approaches for biomarker discovery at trace level.
Selected Peer-Reviewed Publications (48 total) - chronological
1. S. -L Wu, H. Amato, R. Biringer, G. Choudhary, P. Shieh, and W. S. Hancock: "Targeted Proteomics of Low Level Proteins in Human Plasma by LC/MSn: Using Human Growth Hormone as a Model System", J. of Proteome Research. 2002, 1, 459-465.
2. S. -L Wu, W. S. Hancock, G. G. Goodrich, S. T. Kunitake: "A Proteomic Approach to the Detection of Tumor Marker Proteins in a Breast Cancer Cell line (SKBR-3)", Proteomics 2003, (3) 374, issue 6 (June).
3. S. -L. Wu, I. Jardine, W.S. Hancock, and B.L. Karger "A new and sensitive on-line liquid chromatography / mass spectrometric approach for top-down protein analysis: the comprehensive analysis of human growth hormone in an E. coli lysate using a hybrid linear ion trap Fourier transform ion cyclotron resonance mass spectrometer", Rapid Commun. Mass Spectrom. 2004; 18: 2201-2207.
4. S. -L. Wu, JK. Kim, W.S. Hancock, and B.L. Karger "Extended Range Proteomic Analysis (ERPA): A New and Sensitive LC-MS Platform for High Sequence Coverage of Complex Proteins with Extensive Posttranslational Modifications — Comprehensive Analysis of Beta-Casein and Epidermal Growth Factor Receptor (EGFR)", J. of Proteome Research 2005, 4, 1155-1170.
5. S. -L. Wu, JK. Kim, R.W. Bandle, L. Liotta, E. Petricoin, and B.L. Karger "Dynamic profiling of the post-translational modifications and interaction partners of epidermal growth factor receptor signaling after stimulation by EGF using extended range proteomic analysis (ERPA)". Mol. Cell Proteomics, 2006, 5,1610-1627.
6. Y.H. Wang, S.-L. Wu, and W.S. Hancock "Approaches to the Study of N-linked Glycoproteins in Human Plasma Using Lectin Affinity Chromatography and Nano-HPLC Coupled to Electrospray Linear Ion Trap - Fourier Transform Sepctrometry (LTQ-FTMS)", Glycobiology 2006, 16, 514-23.
7. Y.H. Wang, S.-L. Wu, and W.S. Hancock "Monitoring of glycoprotein products in cell culture lysates using lectin affinity chromatography and capillary HPLC coupled to electrospray linear ion trap-Fourier transform mass spectrometry (LTQ/FTMS)". Biotechnol Prog. 2006, 22, 873-80.
8. X.Y. Zheng, S.-L. Wu and W.S. Hancock "Glycation of Interferon-beta-1b and Human Serum Albumin in a Lyophilized Glucose Formulation — Part III: Application of Proteomic Analysis to Manufacturing of Biological Drugs". Int. J. of Pharmaceutics 2006, 322, 136-145.
9. Q. Luo , Y. Yue, G.A,Valaskovic, Y. Gu, S.-L. Wu, B.L. Karger. "On-line 1D and 2D porous layer open tubular/LC-ESI-MS using 10-microm-i.d. poly(styrene-divinylbenzene) columns for ultrasensitive proteomic analysis". Anal Chem. 2007, 79, 6174-81.
10. S.-L. Wu, Y. Gu, D. Hanlon, J. Meyer, J. Linder, W.S. Hancock, B.L. Karger. "Proteomic Analysis of High-Grade Dysplastic Cervical Cells Obtained From ThinPrep® Slides Using Laser Capture Microdissection and Mass Spectrometry", 2007, J Proteome Res. 2007, 6, 4256-68.
11. S. -L. Wu, A. Huhmer, Z.-Q. Hao, and B.L. Karger. "On-Line LC-MS Approach Combining Collision-Induced Dissociation (CID), Electron-Transfer Dissociation (ETD), and CID of an Isolated Charge-Reduced Species for the Trace-Level Characterization of Proteins with Post-Translational Modifications". J Proteome Res. 2007, 6, 4230-44.
12. S.-L. Wu, H. Jiang, Q. Lu, S. Dai, W. S. Hancock, and B. L. Karger. "Mass Spectrometric Determination of Disulfide Linkages in Recombinant Proteins Using On-line LC-MS with Electron Transfer Dissociation (ETD)". 2008, submission.
13. C. Lu, N. Murugesan, J. A. Macdonald, S. –L. Wu*, J. Pachter*, and W. S. Hancock. "Analysis of Mouse Brain Microvascular Endothelium Using Immuno-Laser Capture Microdissection coupled to a hybrid LTQ-FT MS proteomics platform". Electrophoresis, in press, 2008.